Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Plant‐Derived Viral Nanoparticles Enable Simultaneous Guidance of Neuronal Cell Outgrowth and Targeting of Neurodifferentiation Pathways

doi: 10.1002/smll.202509395

Figure Lengend Snippet: Analysis of plant virus nanoparticles displaying functional motifs. A) Purified PVX or TMV VNPs displaying RGD, IKVAV, or BDNF were separated by SDS‐PAGE, and proteins were detected with Coomassie Brilliant Blue or by western blot using anti‐PVX CP or anti‐TMV CP/GAR AP antibodies to detect the ≈30‐kDa modified PVX CP or the ≈20‐kDa TMV CP. B) Electron micrographs of VNPs. Scale bar represents 500 nm. C) Analysis of encapsidated viral RNA.

Article Snippet: The proteins from the cells that were incubated with VNPs carrying laminin‐derived peptides were incubated with mouse anti‐integrin β‐1 (Santa Cruz Biotechnology), and the proteins from the cells incubated with PVX‐BDNF‐2A were labeled with rabbit anti‐Trk (Cell Signaling Technology).

Techniques: Virus, Functional Assay, Purification, SDS Page, Western Blot, Modification

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Plant‐Derived Viral Nanoparticles Enable Simultaneous Guidance of Neuronal Cell Outgrowth and Targeting of Neurodifferentiation Pathways

doi: 10.1002/smll.202509395

Figure Lengend Snippet: Analysis of neurodifferentiation of SH‐SY5Y cells after 22 days. A) Immunostaining of cells with β‐3 tubulin (red) and DAPI‐labeled cell nuclei (blue). Scale bar represents 100 µm. B) Analysis of neuron morphology (average number of neurites; average neurite length; average number of branches); n = 6. C) qPCR analysis of fold expression changes of neuronal markers RNA binding fox‐1 homolog 3 (RBFOX3), Neuronal Cell Adhesion Molecule 1 (NCAM) and Neuron Specific Enolase (NSE); n = 3, p ≤ 0.05 (*), p ≤ 0.001 (**), p ≤ 0.0001 (***), p ≤ 0.00001 (****), statistical analysis: one‐way analysis of variance.

Article Snippet: The proteins from the cells that were incubated with VNPs carrying laminin‐derived peptides were incubated with mouse anti‐integrin β‐1 (Santa Cruz Biotechnology), and the proteins from the cells incubated with PVX‐BDNF‐2A were labeled with rabbit anti‐Trk (Cell Signaling Technology).

Techniques: Immunostaining, Labeling, Expressing, RNA Binding Assay